| Seeded Teams: | Non-Seeded Teams: |
1 Aston Villa | 1 Accrington Stanley |
| 2 Barnsley | 2 Barnet |
| 3 Birmingham City | 3 Brentford |
| 4 Bolton Wanderers | 4 Brighton & Hove Albion |
| 5 Charlton Athletic | 5 Bury |
| 6 Derby County | 6 Carlisle United |
| 7 Everton | 7 Chesterfield |
| 8 Fulham | 8 Cheltenham Town |
| 9 Hull City | 9 Crewe Alexandra |
| 10 Leeds United | 10 Darlington |
| 11 Leicester City | 11 Doncaster Rovers |
| 12 Luton Town | 12 Hartlepool United |
| 13 Manchester City | 13 Hereford United |
| 14 Middlesbrough | 14 Mansfield Town |
| 15 Norwich City | 15 Millwall |
| 16 Portsmouth | 16 Milton Keynes Dons |
| 17 Queens Park Rangers | 17 Notts County |
| 18 Reading | 18 Peterborough United |
| 19 Sheffield United | 19 Port Vale |
| 20 Southampton | 20 Rotherham United |
| 21 Southend United | 21 Scunthorpe United |
| 22 Watford | 22 Walsall |
| 23 West Bromwich Albion | 23 Wrexham |
| 24 Wigan Athletic | 24 Wycombe Wanderers |
The draw will be made at 1.30pm on Sky Sports News 'Soccer Saturday'.